MicroRNA-124 regulates apoptosis in sevoflurane anesthesia-induced neuroblastoma cells by targeting enhancer of zeste homolog 2

Purpose: To investigate the mechanism of microRNA-124 action on neuroblastoma apoptosis induced by sevoflurane.
 Methods: MiR-124 expression was assessed in a cell line (SMS-KAN) using quantitative reverse transcription polymerase chain reaction (qRT-PCR). The role miR-124 sevoflurane anesthesia-induced studied activity and analysis 3-(4, 5-dimethylthiazolyl-2-yl)-2-5 diphenyl tetrazolium bromide (MTT) assay flow cytometry, respectively. target protein genes were confirmed via luciferase reporter activity, qRT-PCR, western blot analysis.
 Results: upregulated (p < 0.05). After knockdown, significantly reduced viability enhanced SMS-KAN nerve cells Furthermore, significant reduction observed 293T co-transfected with mimics EZH2-wild type (EZH2-WT) mRNA levels EZH2 decreased transfected Overexpression inhibited SMSKAN inhibitor offset siEZH2.
 Conclusion: results suggest that overexpression suppresses proliferation targeting cells. Therefore, represents potential for therapy